u133a genechip (Thermo Fisher)
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u133a genechip
U133a Genechip, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u133a+genechips/nct01046825-7-18-18
Average 90 stars, based on 1 article reviews
U133a Genechip, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u133a+genechips/nct01046825-7-18-18
Average 90 stars, based on 1 article reviews
u133a genechip - by Bioz Stars,
2026-09
90/100 stars
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Gene Expression:Article Title: HIF1α-mediated transactivation of WTAP promotes AML cell proliferation via m 6 A-dependent stabilization of KDM4B mRNA. Article Snippet: Hypoxia inducible factor 1α (HIF1α) is abnormally overexpressed in t(8;21) acute myeloid leukemia (AML) and functions as an oncogene through transactivating DNA methyltransferase 3 alpha leading to DNA hypermethylation.. However, it remains unclear whether HIF1α influences RNA N-methyladenosine (mA) methyltransferases.. Here, we show that HIF1α promotes the expression of Wilms tumor 1-associated protein (WTAP), a main component of the mA methyltransferase complex, markedly alters the transcriptome-wide mA distribution and enhances cell proliferation in t(8;21) AML. Article Title: Transcriptional networks in plasmacytoid dendritic cells stimulated with synthetic TLR 7 agonists Article Snippet: .. To this end, we evaluated global gene expression changes after stimulation of human pDC of greater than 99% purity with the TLR7 agonist 3M-852A, and the TLR7/8 agonist 3M-011 using Article Title: Abstract Article Snippet: Methods: Gene expression profiles (GEP) from Affymetrix U133A Genechips were contrasted between HG 1 (low grade) and 3 (high grade) tumours using a training set of 64 estrogen receptor (ER)-positive breast cancer samples. .. Methods: Gene expression profiles (GEP) from Expressing:Article Title: Abstract Article Snippet: Using Affymetrix U133A GeneChips, we have identified 131 up-regulated genes and 156 down-regulated genes whose steady state expression levels differed by at least 3-fold between TAM-r cells and their TAM-sensitive (TAM-s) controls (Scott et al ., submitted). .. Using Article Title: Specific and nontoxic silencing in mammalian cells with expressed long dsRNAs Article Snippet: Biotinylated cRNA was synthesized from total RNA (Enzo, Farmingdale, NY). .. Following processing according to the Affymetrix GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA), the labeled cRNA was hybridized to Synthesized:Article Title: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Article Snippet: .. Briefly, first strand cDNA was synthesized from 5 mg total RNA using a T7-(dT)24 primer (Genset Corporation, La Jolla, CA, USA) and reverse-transcribed using a Superscript DoubleStranded cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was purified, and the subsequent in vitro transcription reaction was performed using a Bioarray Kit (Enzo Life Sciences, Exeter, UK) to generate biotinylated cRNA in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix for cRNA amplification and biotin labeling. cRNA was subsequently fragmented and hybridized to Reverse Transcription:Article Title: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Article Snippet: .. Briefly, first strand cDNA was synthesized from 5 mg total RNA using a T7-(dT)24 primer (Genset Corporation, La Jolla, CA, USA) and reverse-transcribed using a Superscript DoubleStranded cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was purified, and the subsequent in vitro transcription reaction was performed using a Bioarray Kit (Enzo Life Sciences, Exeter, UK) to generate biotinylated cRNA in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix for cRNA amplification and biotin labeling. cRNA was subsequently fragmented and hybridized to cDNA Synthesis:Article Title: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Article Snippet: .. Briefly, first strand cDNA was synthesized from 5 mg total RNA using a T7-(dT)24 primer (Genset Corporation, La Jolla, CA, USA) and reverse-transcribed using a Superscript DoubleStranded cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was purified, and the subsequent in vitro transcription reaction was performed using a Bioarray Kit (Enzo Life Sciences, Exeter, UK) to generate biotinylated cRNA in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix for cRNA amplification and biotin labeling. cRNA was subsequently fragmented and hybridized to Purification:Article Title: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Article Snippet: .. Briefly, first strand cDNA was synthesized from 5 mg total RNA using a T7-(dT)24 primer (Genset Corporation, La Jolla, CA, USA) and reverse-transcribed using a Superscript DoubleStranded cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was purified, and the subsequent in vitro transcription reaction was performed using a Bioarray Kit (Enzo Life Sciences, Exeter, UK) to generate biotinylated cRNA in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix for cRNA amplification and biotin labeling. cRNA was subsequently fragmented and hybridized to Article Title: Image-Based Assessment of Growth and Signaling Changes in Cancer Cells Mediated by Direct Cell-Cell Contact Article Snippet: .. RNA lysis buffer was added into both fractions and RNA was purified using a Qiagen miniRNA kit (Valencia, CA). mRNA samples were prepared and processed for transcriptional profiling using the In Vitro:Article Title: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Article Snippet: .. Briefly, first strand cDNA was synthesized from 5 mg total RNA using a T7-(dT)24 primer (Genset Corporation, La Jolla, CA, USA) and reverse-transcribed using a Superscript DoubleStranded cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was purified, and the subsequent in vitro transcription reaction was performed using a Bioarray Kit (Enzo Life Sciences, Exeter, UK) to generate biotinylated cRNA in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix for cRNA amplification and biotin labeling. cRNA was subsequently fragmented and hybridized to Amplification:Article Title: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Article Snippet: .. Briefly, first strand cDNA was synthesized from 5 mg total RNA using a T7-(dT)24 primer (Genset Corporation, La Jolla, CA, USA) and reverse-transcribed using a Superscript DoubleStranded cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was purified, and the subsequent in vitro transcription reaction was performed using a Bioarray Kit (Enzo Life Sciences, Exeter, UK) to generate biotinylated cRNA in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix for cRNA amplification and biotin labeling. cRNA was subsequently fragmented and hybridized to Labeling:Article Title: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Article Snippet: .. Briefly, first strand cDNA was synthesized from 5 mg total RNA using a T7-(dT)24 primer (Genset Corporation, La Jolla, CA, USA) and reverse-transcribed using a Superscript DoubleStranded cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was purified, and the subsequent in vitro transcription reaction was performed using a Bioarray Kit (Enzo Life Sciences, Exeter, UK) to generate biotinylated cRNA in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix for cRNA amplification and biotin labeling. cRNA was subsequently fragmented and hybridized to Article Title: Specific and nontoxic silencing in mammalian cells with expressed long dsRNAs Article Snippet: Biotinylated cRNA was synthesized from total RNA (Enzo, Farmingdale, NY). .. Following processing according to the Affymetrix GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA), the labeled cRNA was hybridized to Derivative Assay:Article Title: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Article Snippet: .. Briefly, first strand cDNA was synthesized from 5 mg total RNA using a T7-(dT)24 primer (Genset Corporation, La Jolla, CA, USA) and reverse-transcribed using a Superscript DoubleStranded cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was purified, and the subsequent in vitro transcription reaction was performed using a Bioarray Kit (Enzo Life Sciences, Exeter, UK) to generate biotinylated cRNA in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix for cRNA amplification and biotin labeling. cRNA was subsequently fragmented and hybridized to Lysis:Article Title: Image-Based Assessment of Growth and Signaling Changes in Cancer Cells Mediated by Direct Cell-Cell Contact Article Snippet: .. RNA lysis buffer was added into both fractions and RNA was purified using a Qiagen miniRNA kit (Valencia, CA). mRNA samples were prepared and processed for transcriptional profiling using the |
